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Canadian Journal of Anesthesia, Vol 22, 219-223, Copyright © 1975 by Canadian Anesthesiologists' Society
1 Departments of Anaesthesia and Microbiology, University of Manitoba, and Health Sciences Centre, Winnipeg, Manitoba
Using a technique of artificial contamination of anaesthetic breathing tubes we have examined the bacteriological efficacy of a mechanized hot water pasteurization process which involves use of an automatic clothes washing machine. Organisms tested were Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Streptococcus faecalis. Contamination was massive, greater than 108 organisms/ml. Following pasteurization, 88 of 96 breathing tubes showed no growth of the test organisms while recovery of bacteria in the remaining 8 was at the rate of less than 1 organism/40 ml. Pasteurization provides a relatively simple and reliable method of disinfecting some types of anaesthetic equipment, in the absence of other more effective methods.
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