| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Canadian Journal of Anesthesia, Vol 44, 989-995, Copyright © 1997 by Canadian Anesthesiologists' Society
ARTICLES |
CY Li, TC Chou, CS Wong, ST Ho, CC Wu, MH Yen and YA Ding
Department of Anesthesiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China. cyli@ndmcl.ndmctsgh.edu.tw
PURPOSE: To evaluate the effects of ketamine on the activity and protein expression of inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide (LPS) in rat alveolar macrophages. METHODS: Pulmonary alveolar macrophages isolated from Wistar-Kyoto rats were used. After incubation of macrophages with ketamine (1, 10, or 100 microM) and LPS (1 microgram.ml-1) for 24 hr, the cell-free medium was removed for measuring the nitrite and tumour necrosis factor-alpha (TNF-alpha) levels by Griess reaction and ELISA kit, respectively. The harvested macrophages were also used to determine the activity of iNOS by using the conversion of [3H]-L-arginine to [3H]-L-citrulline method. In addition, the protein expression of iNOS was detected by Western blot analysis. RESULTS: In rat alveolar macrophages, (i) ketamine (1 to 100 microM) caused a dose-dependent suppression of the production of nitrite and TNF-alpha induced by LPS and (ii) ketamine (100 microM) inhibited the activity (46.5 +/- 4.8%, P < 0.05) and protein expression (35 +/- 11%, P X 0.05) of iNOS in response to LPS. CONCLUSION: These results show that ketamine inhibits the activity and expression of iNOS in LPS-activated alveolar macrophages, which may be associated with the reduction of the release of TNF-alpha following LPS treatment.
This article has been cited by other articles:
 |
|
 |
|
  J. M. Fuentes, M. A. Talamini, W. B. Fulton, E. J. Hanly, A. R. Aurora, and A. De Maio General Anesthesia Delays the Inflammatory Response and Increases Survival for Mice with Endotoxic Shock Clin. Vaccine Immunol., February 1, 2006; 13(2): 281 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. S. Shibakawa, Y. Sasaki, Y. Goshima, N. Echigo, Y. Kamiya, K. Kurahashi, Y. Yamada, and T. Andoh Effects of ketamine and propofol on inflammatory responses of primary glial cell cultures stimulated with lipopolysaccharide Br. J. Anaesth., December 1, 2005; 95(6): 803 - 810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sun, F. Li, J. Chen, and J. Xu Effect of Ketamine on NF-kappa B Activity and TNF-alpha Production in Endotoxin-Treated Rats Ann. Clin. Lab. Sci., April 1, 2004; 34(2): 181 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yu, Z. Zhou, J. Xu, Z. Liu, and Y. Wang Ketamine Reduces NF{kappa}B Activation and TNF{alpha} Production in Rat Mononuclear Cells Induced by Lipopolysaccharide In Vitro Ann. Clin. Lab. Sci., July 1, 2002; 32(3): 292 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Mullenheim, R. Rulands, T. Wietschorke, J. Fra{beta}dorf, B. Preckel, and W. Schlack Late Preconditioning is Blocked by Racemic Ketamine, But Not by S(+)-Ketamine Anesth. Analg., August 1, 2001; 93(2): 265 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Lewis, B. Rogachev, G. Shaked, and A. Douvdevani The In Vitro Effects of Ketamine at Large Concentrations Can Be Attributed to a Nonspecific Cytostatic Effect Anesth. Analg., April 1, 2001; 92(4): 927 - 929. [Full Text] [PDF]
|
|
|
|
|
|
M. Shiga, K. Nishina, K. Mikawa, and H. Obara The Effects of Lidocaine on Nitric Oxide Production from an Activated Murine Macrophage Cell Line Anesth. Analg., January 1, 2001; 92(1): 128 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kawasaki, M. Ogata, C. Kawasaki, J.-i. Ogata, Y. Inoue, and A. Shigematsu Ketamine Suppresses Proinflammatory Cytokine Production in Human Whole Blood In Vitro Anesth. Analg., September 1, 1999; 89(3): 665 - 665. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
