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The HemoCue®, a point of care B-hemoglobin photometer, measures hemoglobin concentrations accurately when mixed in vitro with canine plasma and three hemoglobin-based oxygen carriers (HBOC)

[Le photomètre de chevet HemoCue® fournit une mesure exacte de concentrations d'hémoglobine combinées in vitro à du plasma canin et à trois transporteurs d'oxygène à base d'hémoglobine]

Jonathan S. Jahr, MD^*, Fedor Lurie, MD PhD, Bernd Driessen, DVM PhD, Jessica A. Davis, Robert Gosselin, MT and Robert A. Gunther, PhD

^* From the Departments of Anesthesiology University of California Los Angeles School of Medicine Charles R. Drew University of Medicine Science Martin Luther King Jr/Drew Medical Center Los Angeles California
The Department Of Clinical Studies New Bolton Center University of Pennsylvania Kennett Square Pennsylvania; and
The Departments Of Anesthesiology Pain Medicine Surgery Medical Pathology University of California-Davis School of Medicine Sacramento California USA.

Dr. J.S. Jahr, Department of Anesthesiology, UC Los Angeles Center for the Health Sciences, Box 951778, Los Angeles, CA 90095, USA. Phone: 310-825-6761; Fax: 310-206-4626; E-mail: jsjahr{at}mednet.ucla.edu

Purpose: Accuracy of measurement of low hemoglobin concentrations using the HemoCue®, a B-hemoglobin photometer (HemoCue AB, Angelholm, Sweden) may exhibit significant variability. Infusion of hemoglobin-based oxygen carriers (HBOC) results in low concentrations of plasma hemoglobin. Our study assessed B-hemoglobin photometer measurement accuracy of three HBOC: (hemoglobin glutamer-200 (bovine; Oxyglobin®, Biopure Corp., Cambridge, MA, USA); hemoglobin glutamer-250 (bovine; Hemopure®, Biopure Corp, Cambridge, MA, USA), and hemoglobin-raffimer, (human; Hemolink^TM, Hemosol, Inc., Toronto, Ontario, Canada).

Methods: In the laboratory, 45 split canine plasma samples were mixed with hemoglobin glutamer-200 (8.13, 16.25, 32.5 g•L^-1 concentrations), 45 samples were mixed with hemoglobin glutamer-250 (8.13, 16.25, 32.5 g•L^-1 concentrations), 45 with hemoglobin-raffimer (12.5, 25.0, 50.0 g•L^-1 concentrations), and measured. Plasma samples without HBOC served as control. Hemoglobin concentration was determined by a laboratory analyzer (Coulter Corporation, Hiafeah, FL, USA) and B-hemoglobin photometer (HemoCue®, Ångelholm, Sweden). Two independent technicians performed blinded sample measurements and randomly tested each sample five times. Results were analyzed according to Bland and Altman analysis.

Results: B-hemoglobin photometer demonstrated high repeatability for all three HBOCs. Repeatability coefficients were 0.37 g•L^-1 and 0.48 g•L^-1 for hemoglobin glutamer-200, 0.39 g•L^-1 and 0.4 g•L^-1 for hemoglobin glutamer-250 and 1.07 g•L^-1 and 0.85 g•L^-1 for hemoglobin-raffimer. An acceptable agreement was found between the B-hemoglobin photometer and the laboratory analyzer for all three HBOCs tested.

Conclusion: The B-hemoglobin photometer accurately determined the concentration of three HBOC solutions dissolved in canine plasma.

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