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Canadian Journal of Anesthesia 49:A80 (2002)
© Canadian Anesthesiologists' Society, 2002

Abstracts - Tuesday June 25th 2002 1030 - 1230

RBC ANTIOXIDANT CAPACITY-PLASMA ISOPROSTANE CORRELATION DURING CPB

David M. Ansley, MD, Zhengyuan Xia, MD, B.S. Dhaliwal, MSc, Thomas K.H. Chang, PhD and David V. Godin, PhD

Centre for Anesthesia & Analgesia, Depts. of Anesthesia, Pharmacology & Therapeutics, and Pharmaceutical Sciences, University of British Columbia, Vancouver, BC

INTRODUCTION

Pharmacologic enhancement of red blood cell (RBC) antioxidant capacity during CPB in humans was associated with improved postoperative cardiac index (1). We investigated whether RBC antioxidant capacity predicts alterations in oxidative stress during CPB in humans.

METHODS

After ethical approval and informed, written consent, 8 patients (age 66 ± 9 yr), for ACBP, were anesthetized with fentanyl and isoflurane. Central venous blood was drawn at pre-induction (T0), after 30 min of CPB (T1), 10 (T2), 30 (T3) and 120 min of reperfusion (T4) to measure RBC antioxidant capacity (malondialdehyde (MDA) production in response to ex vivo forced peroxidation and plasma free isoprostane concentration (radioimmunoassay). Data were expressed as mean ± SEM.

RESULTS

RBC MDA production at 1 mM t-BHP were 93.9 ± 11.5, 101.8 ± 10.1, 104.3 ± 10.4, 99.1 ± 9.1 and 102.5 ± 10.3 nM/ g RBC respectively at T0 , T1 , T2 , T3 and T4. Plasma free isoprostane concentration were 90.4 ± 25.9, 175.2 ± 57.4, 129.5 ± 18.1, 106.0 ± 19.5 and 98.8 ± 23.4 respectively at T0, T1, T2, T3 and T4 . RBC MDA at T1 was significantly correlated with plasma free isoprostane at T1 (R = 0.803, P = 0.0165). RBC MDA at T0, T1, T2 and T3 were all significantly correlated with plasma free [isoprostane] at T3 (R equal to 0.770, 0.829, 0.715 and 0.789 respectively, all P less than 0.05). A correlation of RBC MDA and plasma free [isoprostane] at T0 was not observed (R = 0.561, P = 0.148).

DISCUSSION

During CPB changes in RBC antioxidant capacity predicted changes in the concentration of isoprostane, a specific marker of lipid peroxidation and potent vasoconstrictor. Isoprostane may contribute to postoperative myocardial depression. Alterations in RBC antioxidant capacity may serve as an index in estimating oxidative stress in humans in pathophysiological situations similar to CPB.

REFERENCE

1 Can J Anaesth 46(7):641–8.





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